Modelling Inflammation with iPS-Macrophages and Microglia

Programme details

Four-week Online Course

The online course modules require a time commitment of approximately 4 hours per week:

Week 1 (commencing 20 May 2019)

• Standard human iPS cell culture methods
• Automated, high-throughput culture of iPS cells
• CRISPR/Cas9-mediated gene editing of iPS cells
• Moderated discussion

Week 2 (commencing 27 May 2019)

• Differentiation of macrophages from iPS cells
• Differentiation of microglia-like cells from iPS cells
• Moderated discussion

Week 3 (commencing 3 June 2019)

• Differentiation of cortical neurons from iPS cells
• Differentiation of astrocytes from iPS cells
• Co-culture of microglia, neurons and astrocytes from iPS-derived precursors
• Moderated discussion

Week 4 (commencing 10 June 2019)

• Live cell imaging methodologies for studying cellular dynamics
• Phagocytosis assays
• Profiling cytokine production
• Approaches to developing screens using iPS cells
• Moderated discussion

Practical Course (optional day - limited places available)

Wednesday 19 June 2019

By the end of the optional intensive one-day practical course, which takes place in Oxford, you will have gained hands-on experience of:

• Differentiation of macrophages from iPS cells
• Co-culture of microglia, neurons and astrocytes from iPS-derived precursors
• Downstream assays
• Imaging microglia

Certification

Participants who attend the full course will receive a Certificate of Attendance. The sample shown is an illustration only and the wording will reflect the course and dates attended.

Fees

Online course: £595.00
Online course AND optional practical course: £990.00

Payment

All courses are VAT exempt.

Fees include course materials and tuition.

If you also enrol on the optional practical course: course fee includes lunch on 19 June. The price does not include accommodation.

Tutors

Professor William James

Professor of Virology, Sir William Dunn School of Pathology, University of Oxford

Tutor in Medical Sciences, Brasenose College Oxford

A graduate of the University of Birmingham, William obtained his DPhil in Biochemistry in 1984 at the University of Oxford studying the genetic control of cellular differentiation and respiration in bacteria, during which he cloned the genes for the master transcriptional regulator, gerE, and haem A synthase, CtaA. He was appointed to an academic position at the Dunn School in Oxford on graduation, where he has studied the processes of cellular infection by viruses including HIV-1 and Dengue virus, particularly their adaptations to macrophages. His group were the first to report inhibition of HIV-1 replication by endogenous antisense RNA and by synthetic RNA aptamers, and elucidated the endocytic pathway of HIV-1 infection in macrophages. In order to provide a genetically tractable but physiologically authentic model for human macrophages in vitro, his group developed the pluripotent stem cell model for macrophage differentiation. The Oxford Stem Cell Facility in the James Lab was joined by Dr Cowley in 2007, and with sponsorship from  the James Martin foundation and many others has developed an internationally recognized pipeline in hiPS cell generation, mutagenesis and differentiation.

In addition to research, William has served as Head of Department at the Sir William Dunn School of Pathology, Deputy Head of the University’s Medical Sciences Division, and, between 2011 and 2017, was the Oxford University Pro-Vice Chancellor for Planning and Resources. He currently combines the full-time roles of Professor of Virology in the Dunn School and lead Tutor for Medicine at Brasenose College.

Dr Sally Cowley

Head, James Martin Stem Cell Facility, University of Oxford

A graduate of Natural Sciences at Cambridge, Sally began her research career working on host-pathogen interactions (Ph.D 1990 University of London, and later at AHRI, Ethiopia). Her post-doctoral work (New England Deaconess Hospital, Harvard, Boston, and Institute of Cancer Research, London) centred around signal transduction pathways involved in differentiation: She identified and cloned a novel tyrosine kinase, MATK, implicated in megakaryocyte differentiation; and was the first to demonstrate that the protein kinase MEK (/MKK) is critical for signal transduction pathways leading to differentiation and to tumorigenic transformation.

Following a career break to raise children, she obtained a Wellcome Trust Career Re-entry fellowship and joined the Sir William Dunn School of Pathology in 2007, where, with Professor William James, she established and Heads the James Martin Stem Cell Facility (JMSCF). The Facility has expertise in human induced Pluripotent Stem (iPS) cell derivation, culture, genetic modification and differentiation. iPS cells derived from patients with genetic disease offers a new, hugely exciting opportunity to model human diseases ‘in a dish’. The lab has pioneered methods for differentiation of iPSC to macrophages for modelling innate immunity and infection pathways, thereby overcoming the limitations of blood-derived macrophages. The JMSCF has generated a world-class panel of iPS cells from Parkinson’s patients as part of a large scale Oxford Parkinson’s Disease Centre research programme funded by Parkinson’s UK (in collaboration with Professor Richard Wade-Martins). Sally has been a key member of StemBANCC, leading the development of iPSC gene-editing technologies in this EU-wide collaborative programme focussed on developing iPSc disease models as drug-screening platforms. She is also a Co-Investigator in the MRC DPUK Experimental Medicine Dementia Stem Cell Network, using iPSC-microglia to study neuroinflammation in neurodegenerative disease.  

Learning outcomes

Four-week Online Course

By the end of the online course you will have gained:

• an in depth understanding of the rationale for using human iPS cells to model innate immunity, neuro-inflammation and host-pathogen interactions
• the ability to design strategies for gene knockouts and targeted gene edits in human iPS cells, protocols for carrying out the wet lab work, and an appreciation of potential pitfalls
• an in depth understanding of the practical aspects of culturing human iPS cells at different scales and their differentiation to macrophages, microglia, astrocytes and neurons for monoculture and co-culture
• in depth knowledge of downstream assays relevant to studying macrophage and microglial biology

Practical Course (optional day)

By the end of the practical day in Oxford, you will have gained hands-on experience of:

• handling human iPS cells
• the key manipulations required to make Embryoid Bodies from iPS cells, set Embryoid Bodies up for myelopoiesis differentiation, and harvest of cells from those cultures to plate as macrophages
• setting up iPS-microglia in monoculture and in co-culture for various downstream assays
• imaging iPS-microglia

Application

This course is now full for the current dates in May - June 2019.

We expect to offer this course again later in 2019.

Click the 'Notify me' button on the right if you would like us to contact you about future course dates.

Level and demands

The course is intended for scientists in both Academic labs and in Industry, who will be applying the techniques learnt back in their own laboratories.

Four-week Online Course 

Before you start the online course, you should have some experience in basic mammalian cell culture techniques. It is also helpful if you have any prior experience of working with pluripotent stem cells.

Practical Course (optional day)

If you are hoping to register for the intensive hands-on one-day practical course at Oxford, it is essential that you are competent in basic mammalian cell culture techniques.